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Oak-Associated Negativicute Equipped with Ancestral Aromatic Polyketide Synthase Produces Antimycobacterial Dendrubins.

ABSTRACT: Anaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (Gram-negative staining Firmicutes) such as the oak-associated Dendrosporobacter quercicolus has remained unknown. Genome mining of D. quercicolus and phylogenetic analyses revealed a gene cluster for a type?II polyketide synthase (PKS) complex that belongs to the most ancestral enzyme systems of this type. Metabolic profiling, NMR analyses, and stable-isotope labeling led to the discovery of a new family of anthraquinone-type polyphenols, the dendrubins, which are diversified by acylation, methylation, and dimerization. Dendrubin?A and B were identified as strong antibiotics against a range of clinically relevant, human-pathogenic mycobacteria.

SUBMITTER: Ishida K

PROVIDER: S-EPMC7693217 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Oak-Associated Negativicute Equipped with Ancestral Aromatic Polyketide Synthase Produces Antimycobacterial Dendrubins.

Ishida Keishi K Shabuer Gulimila G Schieferdecker Sebastian S Pidot Sacha J SJ Stinear Timothy P TP Knuepfer Uwe U Cyrulies Michael M Hertweck Christian C

Chemistry (Weinheim an der Bergstrasse, Germany) 20200917 58

Anaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (Gram-negative staining Firmicutes) such as the oak-associated Dendrosporobacter quercicolus has remained unknown. Genome mining of D. quercicolus and phylogenetic analyses revealed a gene cluster for a type II polyketide synthase (PKS) complex that belongs to the most ancestral enzyme systems of this type. Metabolic profiling, NMR analyses, and stable-isotope labeling ...[more]

PMID: 32597507

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