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Laxaphycins B5 and B6 from the cultured cyanobacterium UIC 10484.

ABSTRACT: Two laxaphycin type-B cyclic dodecapeptides, laxaphycins B5 and B6, were obtained from UIC 10484, a freshwater cf. Phormidium sp. Analysis using the 16S rRNA sequence found UIC 10484 to clade with UIC 10045, a known laxaphycin type-A and -B producer, and MS/MS analysis revealed the presence of two novel laxaphycin type-B compounds. The structures of the metabolites were elucidated using 2D NMR and MS/MS. The absolute configurations of the amino acids were determined by advanced Marfey's analysis. Both metabolites were evaluated against the same three cancer cell lines. The IC₅₀ of both laxaphycins B5 and B6 was near 1 μM against breast cancer MDA-MB-231, melanoma MDA-MB-435, and ovarian cancer OVCAR3 cell lines.

SUBMITTER: Sullivan P

PROVIDER: S-EPMC7338234 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Laxaphycins B5 and B6 from the cultured cyanobacterium UIC 10484.

Sullivan Peter P Krunic Aleksej A Burdette Joanna E JE Orjala Jimmy J

The Journal of antibiotics 20200331 8

Two laxaphycin type-B cyclic dodecapeptides, laxaphycins B5 and B6, were obtained from UIC 10484, a freshwater cf. Phormidium sp. Analysis using the 16S rRNA sequence found UIC 10484 to clade with UIC 10045, a known laxaphycin type-A and -B producer, and MS/MS analysis revealed the presence of two novel laxaphycin type-B compounds. The structures of the metabolites were elucidated using 2D NMR and MS/MS. The absolute configurations of the amino acids were determined by advanced Marfey's analysis ...[more]

PMID: 32235860

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