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Sortase A-Inhibitory Metabolites from a Marine-Derived Fungus Aspergillus sp.

ABSTRACT: Seven alkaloidal compounds (2-8) and one polyketide (1) were isolated from a semisolid rice culture of the marine-derived fungus Aspergillus sp. F452. Structures of the isolated compounds were elucidated based on spectroscopic data and comparisons with previously reported data. The alkaloidal compounds (2-8) displayed weak to moderate inhibitory activities against Staphylococcus aureus-derived sortase A (SrtA) without affecting cell viability. Aspermytin A (1) strongly inhibited SrtA activity, with an IC50 value of 146.0 ?M, and significantly reduced bacterial adherence to fibronectin-coated surfaces. The present results indicate that the underlying mechanism of action of compound 1 is associated with the inhibition of SrtA-mediated S. aureus adhesion to fibronectin, thus potentially serving as an SrtA inhibitor.

SUBMITTER: Park SC

PROVIDER: S-EPMC7401278 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Sortase A-Inhibitory Metabolites from a Marine-Derived Fungus Aspergillus sp.

Park Sung Chul SC Chung Beomkoo B Lee Jayho J Cho Eunji E Hwang Ji-Yeon JY Oh Dong-Chan DC Shin Jongheon J Oh Ki-Bong KB

Marine drugs 20200713 7

Seven alkaloidal compounds (2-8) and one polyketide (1) were isolated from a semisolid rice culture of the marine-derived fungus Aspergillus sp. F452. Structures of the isolated compounds were elucidated based on spectroscopic data and comparisons with previously reported data. The alkaloidal compounds (2-8) displayed weak to moderate inhibitory activities against Staphylococcus aureus-derived sortase A (SrtA) without affecting cell viability. Asp ...[more]

PMID: 32668629

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