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Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor.

ABSTRACT: Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 ppm, the Environmental Protection Agency's most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.

SUBMITTER: Thavarajah W

PROVIDER: S-EPMC7412506 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Point-of-Use Detection of Environmental Fluoride <i>via</i> a Cell-Free Riboswitch-Based Biosensor.

Thavarajah Walter W Silverman Adam D AD Verosloff Matthew S MS Kelley-Loughnane Nancy N Jewett Michael C MC Lucks Julius B JB

ACS synthetic biology 20191220 1

Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term ...[more]

PMID: 31829623

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