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Increasing the copper sensitivity of microorganisms by restricting iron supply, a strategy for bio-management practices.


ABSTRACT: Pollution by copper (Cu2+ ) extensively used as antimicrobial in agriculture and farming represents a threat to the environment and human health. Finding ways to make microorganisms sensitive to lower metal concentrations could help decreasing the use of Cu2 + in agriculture. In this respect, we showed that limiting iron (Fe) uptake makes bacteria much more susceptible to Cu2 + or Cd2+ poisoning. Using efflux mutants of the purple bacterium Rubrivivax gelatinosus, we showed that Cu+ and Cd2+ resistance relies on the expression of the Fur-regulated FbpABC and Ftr iron transporters. To support this conclusion, inactivation of these Fe-importers in the Cu+ or Cd2+ -ATPase efflux mutants gave rise to hypersensitivity towards these ions. Moreover, in metal overloaded cells the expression of FbpA, the periplasmic iron-binding component of the ferric ion transport FbpABC system was induced, suggesting that cells perceived an 'iron-starvation' situation and responded to it by inducing Fe-importers. In this context, the Fe-Sod activity increased in response to Fe homoeostasis dysregulation. Similar results were obtained for Vibrio cholerae and Escherichia coli, suggesting that perturbation of Fe-homoeostasis by metal excess appeared as an adaptive response commonly used by a variety of bacteria. The presented data support a model in which metal excess induces Fe-uptake to support [4Fe-4S] synthesis and thereby induce ROS detoxification system.

SUBMITTER: Steunou AS 

PROVIDER: S-EPMC7415376 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Increasing the copper sensitivity of microorganisms by restricting iron supply, a strategy for bio-management practices.

Steunou Anne Soisig AS   Bourbon Marie-Line ML   Babot Marion M   Durand Anne A   Liotenberg Sylviane S   Yamaichi Yoshiharu Y   Ouchane Soufian S  

Microbial biotechnology 20200619 5


Pollution by copper (Cu<sup>2+</sup> ) extensively used as antimicrobial in agriculture and farming represents a threat to the environment and human health. Finding ways to make microorganisms sensitive to lower metal concentrations could help decreasing the use of Cu<sup>2</sup> <sup>+</sup> in agriculture. In this respect, we showed that limiting iron (Fe) uptake makes bacteria much more susceptible to Cu<sup>2</sup> <sup>+</sup> or Cd<sup>2+</sup> poisoning. Using efflux mutants of the purple  ...[more]

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