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Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.

ABSTRACT: Stem cell-based transplantation therapies offer hope for currently untreatable retinal degenerations; however, preclinical progress has been largely confined to rodent models. Here, we describe an experimental platform for accelerating photoreceptor replacement therapy in the nonhuman primate, which has a visual system much more similar to the human. We deployed fluorescence adaptive optics scanning light ophthalmoscopy (FAOSLO) to noninvasively track transplanted photoreceptor precursors over time at cellular resolution in the living macaque. Fluorescently labeled photoreceptors generated from a CRX+/tdTomato human embryonic stem cell (hESC) reporter line were delivered subretinally to macaques with normal retinas and following selective ablation of host photoreceptors using an ultrafast laser. The fluorescent reporter together with FAOSLO allowed transplanted photoreceptor precursor survival, migration, and neurite formation to be monitored over time in vivo. Histological examination suggested migration of photoreceptor precursors to the outer plexiform layer and potential synapse formation in ablated areas in the macaque eye.

SUBMITTER: Aboualizadeh E

PROVIDER: S-EPMC7419740 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.

Aboualizadeh Ebrahim E Phillips M Joseph MJ McGregor Juliette E JE DiLoreto David A DA Strazzeri Jennifer M JM Dhakal Kamal R KR Bateman Brittany B Jager Lindsey D LD Nilles Kelsy L KL Stuedemann Sara A SA Ludwig Allison L AL Hunter Jennifer J JJ Merigan William H WH Gamm David M DM Williams David R DR

Stem cell reports 20200723 2

Stem cell-based transplantation therapies offer hope for currently untreatable retinal degenerations; however, preclinical progress has been largely confined to rodent models. Here, we describe an experimental platform for accelerating photoreceptor replacement therapy in the nonhuman primate, which has a visual system much more similar to the human. We deployed fluorescence adaptive optics scanning light ophthalmoscopy (FAOSLO) to noninvasively track transplanted photoreceptor precursors over t ...[more]

PMID: 32707075

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Dataset Information

Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.

Publications

Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.

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