Ontology highlight
ABSTRACT:
SUBMITTER: Bramlett C
PROVIDER: S-EPMC7427513 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Bramlett Charles C Jiang Du D Nogalska Anna A Eerdeng Jiya J Contreras Jorge J Lu Rong R
Nature protocols 20200304 4
Embedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing dat ...[more]