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Clonal tracking using embedded viral barcoding and high-throughput sequencing.

ABSTRACT: Embedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data. The entire procedure can be completed within a few weeks. This barcoding method requires cells to be susceptible to viral transduction. It provides high sensitivity and throughput, and enables precise quantification of cellular progeny. It is cost efficient and does not require any advanced skills. It can also be easily adapted to many types of applications, including both in vitro and in vivo experiments.

SUBMITTER: Bramlett C

PROVIDER: S-EPMC7427513 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Clonal tracking using embedded viral barcoding and high-throughput sequencing.

Bramlett Charles C Jiang Du D Nogalska Anna A Eerdeng Jiya J Contreras Jorge J Lu Rong R

Nature protocols 20200304 4

Embedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing dat ...[more]

PMID: 32132718

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