Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.
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ABSTRACT:
SUBMITTER: Yu J
PROVIDER: S-EPMC7439560 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.
Yu Jinzhu J Oh Ki K Moorthi Sitapriya S Li Ling L Strey Helmut H HH Schuster Michael M Luberto Chiara C Quan Phenix-Lan PL Brouzes Eric E
Lab on a chip 20200301 5
We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry. ...[more]
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2014-06-03 | GSE48294 | GEO