Assessment of calcium oxalate crystal inhibition potential, antioxidant activity and amino acid profiling in horse gram (Macrotyloma uniflorum): high altitude farmer's varieties.

ABSTRACT: Seeds of Macrotyloma uniflorum collected from different Himalayan regions of Himachal Pradesh, India were screened for inhibition potential of calcium oxalate crystals along with their antioxidant and amino acid profiling. Aqueous, ethanol, 50% ethanol extracts were tested using agar-gel overlay, nucleation, and artificial urine methods in comparison to marketed products. The aqueous extract of Sundernagar and Rampur seeds showed highest inhibition potential (9.0 ± 0.81 mm) than alcohol and hydroalcohol. Sundernagar seeds (aqueous extract) also showed maximum inhibition (45 ± 1.2 and 24 ± 0.88%) in nucleation and artificial urine assay, respectively. The Tris-buffer (pH. 8.0) extract of dry seeds showed more inhibition with low protein than high protein germinated seeds. The finding

SUBMITTER: Gautam M 

PROVIDER: S-EPMC7443191 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature