MicroRNA?15a?5p?targeting oncogene YAP1 inhibits cell viability and induces cell apoptosis in cervical cancer cells.

ABSTRACT: MicroRNAs (miRNAs) have been reported to have important regulatory roles in the progression of several types of cancer, including cervical cancer (CC). However, the biological roles and regulatory mechanisms of miRNAs in CC remain to be fully elucidated. The aim of the present study was to examine the functions of miRNAs in CC and the possible mechanisms. Using a microarray, it was identified that miRNA?15a?5p (miR?15a?5p) was one of the most downregulated miRNAs in CC tissues compared with adjacent noncancerous tissues. The low expression of miR?15a?5p was observed in CC tumor tissues with distant metastasis and in CC cell lines. In addition, the effects of miR?15a?5p upregulation on cell viability, apoptosis, invasion and migration of CC cells were investigated using CCK?8, flow cytometry, Transwell and wound healing assays, respectively. It was demonstrated that upregulation of miR?15a?5p significantly suppressed the viability, migration and invasion, and promoted the apoptosis of SiHa and C?33A cells. Furthermore, yes?associated protein 1 (YAP1), a well?known oncogene, was confirmed to be directly targeted by miR?15a?5p and was found to be negatively regulated by miR?15a?5p. Further correlation analysis indicated that miR?15a?5p expression was negatively correlated with YAP1 expression in CC tissues. Notably, overexpression of YAP1 abrogated the tumor suppressive effects of miR?15a?5p in CC cells. Taken together, these present findings indicated that the miR?15a?5p/YAP1 axis may provide a novel strategy for the clinical treatment of CC.

SUBMITTER: Chen X 

PROVIDER: S-EPMC7447307 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature