Dataset Information

Oxidative Stress Response Is Mediated by Overexpression and Spatiotemporal Regulation of Caveolin-1.

ABSTRACT: Oxidative stress (OS) has been linked to the aetiology of many diseases including osteoarthritis (OA). Recent studies have shown that caveolin-1-a structural protein of plasma membrane's caveolae-is upregulated in response to OS. Here, we explore the function of caveolin-1 in chondrocytes derived from healthy individuals (control) and OA patients that were subjected to exogenous OS. We showed that caveolin-1 was upregulated in response to acute OS in the control, but not in OA chondrocytes. Moreover, OS-induced DNA damage analysis revealed that control cells started repairing the DNA lesions 6 h post-oxidative treatment, while OA cells seemed unable to restore these damages. Importantly, in the control cells, we observed a translocation of caveolin-1 from the membrane/cytoplasm in and out of the nucleus, which coincided with the appearance and restoration of DNA lesions. When caveolin-1 was prevented from translocating to the nucleus, the control cells were unable to repair DNA damage. In OA cells, no such translocation of caveolin-1 was observed, which could account for their inability to repair DNA damage. Taken together, these results provide novel insights considering the role of caveolin-1 in response to OS-induced DNA damage while revealing its implication in the pathophysiology of OA.

SUBMITTER: Goutas A

PROVIDER: S-EPMC7464519 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Oxidative Stress Response Is Mediated by Overexpression and Spatiotemporal Regulation of Caveolin-1.

Goutas Andreas A Papathanasiou Ioanna I Mourmoura Evanthia E Tsesmelis Konstantinos K Tsezou Aspasia A Trachana Varvara V

Antioxidants (Basel, Switzerland) 20200818 8

Oxidative stress (OS) has been linked to the aetiology of many diseases including osteoarthritis (OA). Recent studies have shown that caveolin-1-a structural protein of plasma membrane's caveolae-is upregulated in response to OS. Here, we explore the function of caveolin-1 in chondrocytes derived from healthy individuals (control) and OA patients that were subjected to exogenous OS. We showed that caveolin-1 was upregulated in response to acute OS in the control, but not in OA chondrocytes. More ...[more]

PMID: 32824727

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Dataset Information

Oxidative Stress Response Is Mediated by Overexpression and Spatiotemporal Regulation of Caveolin-1.

Publications

Oxidative Stress Response Is Mediated by Overexpression and Spatiotemporal Regulation of Caveolin-1.

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