Ontology highlight
ABSTRACT:
SUBMITTER: Brautigam CA
PROVIDER: S-EPMC7466939 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Brautigam Chad A CA Zhao Huaying H Vargas Carolyn C Keller Sandro S Schuck Peter P
Nature protocols 20160407 5
Isothermal titration calorimetry (ITC) is a powerful and widely used method to measure the energetics of macromolecular interactions by recording a thermogram of differential heating power during a titration. However, traditional ITC analysis is limited by stochastic thermogram noise and by the limited information content of a single titration experiment. Here we present a protocol for bias-free thermogram integration based on automated shape analysis of the injection peaks, followed by combinat ...[more]