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Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions.

ABSTRACT: One of the many ways by which bacteria control gene expression is through cis-acting regulatory mRNA elements called riboswitches. By specifically binding to small molecules or metabolites and pairing the binding event to an RNA structure change, riboswitches link a metabolic input to a transcriptional or translational output. For over a decade, isothermal titration calorimetry (ITC) has been used to investigate how riboswitches interact with small molecules. We present methods for assaying RNA-ligand interactions using ITC and analyzing resulting data to estimate thermodynamic parameters associated with binding.

SUBMITTER: Jones CP

PROVIDER: S-EPMC7324066 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions.

Jones Christopher P CP Piszczek Grzegorz G Ferré-D'Amaré Adrian R AR

Methods in molecular biology (Clifton, N.J.) 20190101

One of the many ways by which bacteria control gene expression is through cis-acting regulatory mRNA elements called riboswitches. By specifically binding to small molecules or metabolites and pairing the binding event to an RNA structure change, riboswitches link a metabolic input to a transcriptional or translational output. For over a decade, isothermal titration calorimetry (ITC) has been used to investigate how riboswitches interact with small molecules. We present methods for assaying RNA- ...[more]

PMID: 30929236

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