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Analysis of cooperativity by isothermal titration calorimetry.

ABSTRACT: Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.

SUBMITTER: Brown A

PROVIDER: S-EPMC2812830 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Analysis of cooperativity by isothermal titration calorimetry.

Brown Alan A

International journal of molecular sciences 20090804 8

Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of coopera ...[more]

PMID: 20111687

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