Ontology highlight
ABSTRACT:
SUBMITTER: Yang JH
PROVIDER: S-EPMC7468127 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Yang Jay-How JH Williams Dewight D Kandiah Eaazhisai E Fromme Petra P Chiu Po-Lin PL
Communications biology 20200902 1
In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the redox modulation, we used single-particle cryo-EM to determine the structures of spinach chloroplast ATP synthase in both reduced and oxidized states. The disulfide linkage of the oxidized γ subunit introduces a torsional constraint to stabilize the two β hairpin structures. Once reduced, free cysteines a ...[more]