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MiR-422a in gastric cancer cells directly targets CDC40 and modulates cell proliferation.

ABSTRACT: MicroRNAs have been shown to be involved in a variety of different human cancers, including gastric cancer, functioning as post-transcriptional regulators of oncogenes or tumor suppressors. This study aimed to clarify the role of miR-422a in gastric cancer and further elucidate the pathogenesis thereof. To this end, miR-422a expression was initially determined in gastric cancer tissues and cells. Our results showed decreased miR-422a and increased cell division cycle 40 (CDC40) expression in gastric cancer. Dual-luciferase reporter assay further confirmed that miR-422a targeted CDC40. Altogether, this study showed that miR-422a downregulated CDC40, thereby affecting cell cycle progression. Moreover, restoration of miR-422a inhibited gastric cancer cell proliferation. In summary, this study has been the first to show that miR-422a was associated with CDC40 levels in human gastric cancer cells and that disease development may be attributed to CDC40.

SUBMITTER: Zhu F

PROVIDER: S-EPMC7476107 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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MiR-422a in gastric cancer cells directly targets CDC40 and modulates cell proliferation.

Zhu Fanglai F Li Hao H Ding Fei F Guo Hao H Mou Hong H Ma Jie J

American journal of translational research 20200815 8

MicroRNAs have been shown to be involved in a variety of different human cancers, including gastric cancer, functioning as post-transcriptional regulators of oncogenes or tumor suppressors. This study aimed to clarify the role of miR-422a in gastric cancer and further elucidate the pathogenesis thereof. To this end, miR-422a expression was initially determined in gastric cancer tissues and cells. Our results showed decreased miR-422a and increased cell division cycle 40 (CDC40) expression in gas ...[more]

PMID: 32913542

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