Dataset Information

Effects of Caponization on Expression of Gonadotropin-Releasing Hormone-I and Gonadotropin Subunits Genes in Roosters.

ABSTRACT: We evaluated the effects of caponization on mRNA levels of gonadotropin-releasing hormone-I (GnRH-I), gonadotropin subunit and other hypothalamic and hypophyseal peptide genes in male chicken. Thirty roosters (25 d) with similar weight were equally divided into the experimental (capons) and control (sham-operated males) groups randomly. Caponization was performed at 28 days of age and birds were slaughtered at 140 days of age. Caponization resulted in increasing levels of luteinizing hormone ? (LH?) and follicle-stimulating hormone ? (FSH?) mRNA in the pituitary gland and levels of LH and FSH in serum (P<0.05 or P<0.01). There were no significant differences in levels of GnRH-I, Gonadotropin releasing hormone receptor (GnRHR), neuropeptide Y (NPY) and Proopiomelanocortin (POMC) mRNA between the two groups. Capons exhibited lower levels of follistatin (FS), estrogen receptor ? (ER?) and higher levels of androgen receptor (AR) mRNA in the pituitary gland compared with sham-operated males (P<0.05). These results suggest that increased LH and FSH concentrations in serum and LH? and FSH? mRNA levels in pituitary after castration are not depended on GnRH synthesis. And changed expression of ER?, AR and FS genes in the pituitary gland may be important components of regulating gonadotropin in capons.

SUBMITTER: Guo X

PROVIDER: S-EPMC7477247 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Effects of Caponization on Expression of Gonadotropin-Releasing Hormone-I and Gonadotropin Subunits Genes in Roosters.

Guo Xing X Zhou Bangyuan B Wan Yi Y Zhou Jun J Shi Dan D Geng Zhaoyu Z Chen Xingyong X Jiang Runshen R

The journal of poultry science 20160101 1

We evaluated the effects of caponization on mRNA levels of gonadotropin-releasing hormone-I (<i>GnRH-I</i>), gonadotropin subunit and other hypothalamic and hypophyseal peptide genes in male chicken. Thirty roosters (25 d) with similar weight were equally divided into the experimental (capons) and control (sham-operated males) groups randomly. Caponization was performed at 28 days of age and birds were slaughtered at 140 days of age. Caponization resulted in increasing levels of luteinizing horm ...[more]

PMID: 32908365

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Project description:BackgroundThe main objective of the present work was to compare the effects of the gonadotropin-releasing hormone agonist (GnRH-a) and GnRH antagonist (GnRH-ant) on the gene expression profiles of oocytes obtained from Iranian infertile couples undergoing in vitro fertilization (IVF).MethodsFifty infertile couples who underwent IVF between June 2012 and November 2013 at the Infertility Center of Tehran Women General Hospital, Tehran University of Medical Sciences, were included in this study. We included women that had undergone IVF treatment because of male factor, tubal factor, or unexplained infertility. The women randomly underwent controlled ovarian stimulation (COS) with either the GnRH-a (n = 26) or the GnRH-ant (n = 24). We obtained 50 germinal vesicle (GV) oocytes donated by women in each group. After the sampling, pool of 50 GV oocytes for each group was separately analyzed by quantitative polymerase chain reaction (qPCR).ResultThe expression levels of Adenosine triphosphatase 6 (ATPase 6), Bone morphogenetic protein 15 (BMP15), and Neuronal apoptosis inhibitory protein (NAIP) genes were significantly upregulated in the GnRH-ant group compared to the GnRH-a group, with the fold change of 3.990 (SD ± 1.325), 6.274 (SD ± 1.542), and 2.156 (SD ± 1.443), respectively, (P < 0.001). Growth differentiation factor 9 (GDF9) mRNA did not have any expression in the GnRH-a group; however, GDF9 mRNA was expressed in the GnRH-ant group. Finally, it was found that the genes involved in the DNA repairing and cell cycle checkpoint did not have any expression in either group.ConclusionThe present study showed, for the first time, the expression levels of genes involved in the cytoplasmic maturity (BMP15, GDF9), adenosine triphosphate production (ATPase 6), and antiapoptotic process (NAIP), in human GV oocytes were significantly higher in the GnRH-anta group than in the GnRH-a group in COS. Higher expression level of these genes when GnRH-ant protocol is applied, this protocol seems to be a more appropriate choice for women with poly cystic ovarian syndrome, because it can probably improve the expression of the aforementioned genes.Trial registrationCurrent Controlled Trials: IRCT 2014031112307 N3.

Project description:The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH forms that are classified into three main groups, namely GnRH1, GnRH2, and GnRH3. In order to gain more insights into the GnRH gene family in vertebrates, we sought to identify which paralogs of this family are present in cartilaginous fish. For this purpose, we searched the genomes and/or transcriptomes of three representative species of this group, the small-spotted catshark, Scyliorhinus canicula, the whale shark, Rhincodon typus and the elephant shark Callorhinchus milii. In each species, we report the identification of three GnRH genes. In catshark and whale shark, phylogenetic and synteny analysis showed that these three genes correspond to GnRH1, GnRH2, and GnRH3. In both species, GnRH1 was found to encode a novel form of GnRH whose primary structure was determined as follows: QHWSFDLRPG. In elephant shark, the three genes correspond to GnRH1a and GnRH1b, two copies of the GnRH1 gene, plus GnRH2. 3D structure prediction of the chondrichthyan GnRH-associated peptides (GAPs) revealed that catshark GAP1, GAP2, and elephant shark GAP2 peptides exhibit a helix-loop-helix (HLH) structure. This structure observed for many osteichthyan GAP1 and GAP2, may convey GAP biological activity. This HLH structure could not be observed for elephant shark GAP1a and GAP1b. As for all other GAP3 described so far, no typical 3D HLH structure was observed for catshark nor whale shark GAP3. RT-PCR analysis revealed that GnRH1, GnRH2, and GnRH3 genes are differentially expressed in the catshark brain. GnRH1 mRNA appeared predominant in the diencephalon while GnRH2 and GnRH3 mRNAs seemed to be most abundant in the mesencephalon and telencephalon, respectively. Taken together, our results show that the GnRH gene repertoire of the vertebrate ancestor was entirely conserved in the chondrichthyan lineage but that the GnRH3 gene was probably lost in holocephali. They also suggest that the three GnRH neuronal systems previously described in the brain of bony vertebrates are also present in cartilaginous fish.

Dataset Information

Effects of Caponization on Expression of Gonadotropin-Releasing Hormone-I and Gonadotropin Subunits Genes in Roosters.

Publications

Effects of Caponization on Expression of Gonadotropin-Releasing Hormone-I and Gonadotropin Subunits Genes in Roosters.

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