Dataset Information

KmerGO: A Tool to Identify Group-Specific Sequences With k-mers.

ABSTRACT: Capturing group-specific sequences between two groups of genomic/metagenomic sequences is critical for the follow-up identifications of singular nucleotide variants (SNVs), gene families, microbial species or other elements associated with each group. A sequence that is present, or rich, in one group, but absent, or scarce, in another group is considered a "group-specific" sequence in our study. We developed a user-friendly tool, KmerGO, to identify group-specific sequences between two groups of genomic/metagenomic long sequences or high-throughput sequencing datasets. Compared with other tools, KmerGO captures group-specific k-mers (k up to 40 bps) with much lower requirements for computing resources in much shorter running time. For a 1.05 TB dataset (.fasta), it takes KmerGO about 21.5 h (including k-mer counting) to return assembled group-specific sequences on a regular stand-alone workstation with no more than 1 GB memory. Furthermore, KmerGO can also be applied to capture trait-associated sequences for continuous-trait. Through multi-process parallel computing, KmerGO is implemented with both graphic user interface and command line on Linux and Windows free from any pre-installed supporting environments, packages, and complex configurations. The output group-specific k-mers or sequences from KmerGO could be the inputs of other tools for the downstream discovery of biomarkers, such as genetic variants, species, or genes. KmerGO is available at https://github.com/ChnMasterOG/KmerGO.

SUBMITTER: Wang Y

PROVIDER: S-EPMC7477287 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

KmerGO: A Tool to Identify Group-Specific Sequences With <i>k</i>-mers.

Wang Ying Y Chen Qi Q Deng Chao C Zheng Yiluan Y Sun Fengzhu F

Frontiers in microbiology 20200825

Capturing group-specific sequences between two groups of genomic/metagenomic sequences is critical for the follow-up identifications of singular nucleotide variants (SNVs), gene families, microbial species or other elements associated with each group. A sequence that is present, or rich, in one group, but absent, or scarce, in another group is considered a "group-specific" sequence in our study. We developed a user-friendly tool, KmerGO, to identify group-specific sequences between two groups of ...[more]

PMID: 32983048

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Project description:Comparing metagenomic samples is crucial for understanding microbial communities. For different groups of microbial communities, such as human gut metagenomic samples from patients with a certain disease and healthy controls, identifying group-specific sequences offers essential information for potential biomarker discovery. A sequence that is present, or rich, in one group, but absent, or scarce, in another group is considered "group-specific" in our study. Our main purpose is to discover group-specific sequence regions between control and case groups as disease-associated markers. We developed a long k-mer (k ? 30 bps)-based computational pipeline to detect group-specific sequences at strain resolution free from reference sequences, sequence alignments, and metagenome-wide de novo assembly. We called our method MetaGO: Group-specific oligonucleotide analysis for metagenomic samples. An open-source pipeline on Apache Spark was developed with parallel computing. We applied MetaGO to one simulated and three real metagenomic datasets to evaluate the discriminative capability of identified group-specific markers. In the simulated dataset, 99.11% of group-specific logical 40-mers covered 98.89% disease-specific regions from the disease-associated strain. In addition, 97.90% of group-specific numerical 40-mers covered 99.61 and 96.39% of differentially abundant genome and regions between two groups, respectively. For a large-scale metagenomic liver cirrhosis (LC)-associated dataset, we identified 37,647 group-specific 40-mer features. Any one of the features can predict disease status of the training samples with the average of sensitivity and specificity higher than 0.8. The random forests classification using the top 10 group-specific features yielded a higher AUC (from ?0.8 to ?0.9) than that of previous studies. All group-specific 40-mers were present in LC patients, but not healthy controls. All the assembled 11 LC-specific sequences can be mapped to two strains of Veillonella parvula: UTDB1-3 and DSM2008. The experiments on the other two real datasets related to Inflammatory Bowel Disease and Type 2 Diabetes in Women consistently demonstrated that MetaGO achieved better prediction accuracy with fewer features compared to previous studies. The experiments showed that MetaGO is a powerful tool for identifying group-specific k-mers, which would be clinically applicable for disease prediction. MetaGO is available at https://github.com/VVsmileyx/MetaGO.

Dataset Information

KmerGO: A Tool to Identify Group-Specific Sequences With k-mers.

Publications

KmerGO: A Tool to Identify Group-Specific Sequences With <i>k</i>-mers.

Similar Datasets

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