Unknown

Dataset Information

0

Evaluation of lymphocyte apoptosis in patients with oral cancer.


ABSTRACT: OBJECTIVES:To evaluate apoptotic levels of peripheral blood mononuclear cells (PBMCs) and apoptotic regulatory proteins (Bax and Bcl-2) in lymphocyte subsets of oral cancer (OC) patients and healthy controls (HC). METHODOLOGY:The percentage of apoptotic cells and lymphocyte counts were measured in the first cohort using PBMCs obtained from 23 OC patients and 6 HC. In the second cohort, (OC, 33; HC, 13), the mean fluorescence intensity (MFI) of Bax and Bcl-2 in CD19+ B, CD4+ T, CD8+ T, and CD16+56+ natural killer (NK) cells was determined via flow cytometry. RESULTS:The percentage of apoptotic cells was higher in the PBMCs of OC patients than in HC patients, particularly in patients with stage IV cancer (p<0.05). However, lymphocyte counts were significantly lower in stage IV patients (p<0.05). NK CD19+ B and CD16+56+ cell counts were significantly lower in OC patients compared with HC patients (p<0.001 and p<0.01, respectively), but CD4+ T cells were interestingly significantly higher in OC patients (p<0.001). While Bax MFI was slightly higher, Bcl-2 MFI was significantly lower for all four lymphocyte subsets in OC samples, particularly in stage IV patients, when compared with HC. Consequently, Bax/Bcl-2 ratios showed an upward trend from HC to OC patients, particularly those in stage IV. We found similar trends in Bax and Bcl-2 MFI for tumor stage, tumor size, and lymph node involvement. CONCLUSIONS:The increased lymphocyte apoptosis in stage IV OC patients may be related to higher Bax levels and lower Bcl-2 levels. The Bax/Bcl-2 ratio in lymphocytes may be useful to determine the prognosis of OC patients, and could be considered a mean for supportive treatment in the future.

SUBMITTER: Bin-Alee F 

PROVIDER: S-EPMC7480670 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Evaluation of lymphocyte apoptosis in patients with oral cancer.

Bin-Alee Fardeela F   Arayataweegool Areeya A   Buranapraditkun Supranee S   Mahattanasakul Patnarin P   Tangjaturonrasme Napadon N   Mutirangura Apiwat A   Kitkumthorn Nakarin N  

Journal of applied oral science : revista FOB 20200907


<h4>Objectives</h4>To evaluate apoptotic levels of peripheral blood mononuclear cells (PBMCs) and apoptotic regulatory proteins (Bax and Bcl-2) in lymphocyte subsets of oral cancer (OC) patients and healthy controls (HC).<h4>Methodology</h4>The percentage of apoptotic cells and lymphocyte counts were measured in the first cohort using PBMCs obtained from 23 OC patients and 6 HC. In the second cohort, (OC, 33; HC, 13), the mean fluorescence intensity (MFI) of Bax and Bcl-2 in CD19+ B, CD4+ T, CD8  ...[more]

Similar Datasets

| S-EPMC5689685 | biostudies-other
| S-EPMC4002783 | biostudies-other
| S-EPMC5322526 | biostudies-literature
| S-EPMC2714500 | biostudies-literature
| S-EPMC8509431 | biostudies-literature
2022-10-08 | E-MTAB-12205 | biostudies-arrayexpress
2022-09-30 | E-MTAB-12202 | biostudies-arrayexpress
| S-EPMC6135683 | biostudies-literature
| S-EPMC3032328 | biostudies-literature
| PRJNA595787 | ENA