Dataset Information

Viscoelastic Properties of ECM-Rich Embryonic Microenvironments.

ABSTRACT: The material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the viscoelastic properties of embryonic tissues. Quail embryos were cultured in a plastic incubator chamber located at the center of two pairs of crossed electromagnets. We found a pronounced viscoelastic behavior within the ECM-rich region separating the mesoderm and endoderm in Hamburger Hamilton stage 10 quail embryos, consistent with a Zener (standard generalized solid) model. The viscoelastic response is about 45% of the total response, with a characteristic relaxation time of 1.3 s.

SUBMITTER: Akos Z

PROVIDER: S-EPMC7487363 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Viscoelastic Properties of ECM-Rich Embryonic Microenvironments.

Akos Zsuzsa Z Isai Dona Greta DG Rajasingh Sheeja S Kosa Edina E Ghazvini Saba S Dhar Prajnaparamita P Czirok Andras A

Frontiers in cell and developmental biology 20200831

The material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the viscoelastic properties of embryonic tissues. Quail embryos were cultured in a plastic incubator chamber located at the center of two pairs of crossed electromagnets. We found a pronounced viscoelastic be ...[more]

PMID: 32984301

Similar Datasets

Project description:Pancreatic ductal adenocarcinoma (PDAC) is almost universally fatal, in large part due to a protective fibrotic barrier generated by tumor-associated stromal (TAS) cells. This barrier is thought to promote cancer cell survival and confounds attempts to develop effective therapies. We present a 3D in vitro system that replicates the mechanical properties of the PDAC microenvironment, representing an invaluable tool for understanding the biology of the disease. Mesoscale indentation quantified viscoelastic metrics of resected malignant tumors, inflamed chronic pancreatitis regions, and histologically normal tissue. Both pancreatitis (2.15?±?0.41?kPa, Mean?±?SD) and tumors (5.46?±?3.18?kPa) exhibit higher Steady-State Modulus (SSM) than normal tissue (1.06?±?0.25?kPa; p?<?.005). The average viscosity of pancreatitis samples (63.2?±?26.7?kPa·s) is significantly lower than that of both normal tissue (252?±?134?kPa·s) and tumors (349?±?222?kPa·s; p?<?.005). To mimic this remodeling behavior, PDAC and TAS cells were isolated from human PDAC tumors. Conditioned medium from PDAC cells was used to culture TAS-embedded collagen hydrogels. After 7?days, TAS-embedded gels in control medium reached SSM (1.45?±?0.12?kPa) near normal pancreas, while gels maintained with conditioned medium achieved higher SSM (3.38?±?0.146?kPa) consistent with tumors. Taken together, we have demonstrated an in vitro system that recapitulates in vivo stiffening of PDAC tumors. In addition, our quantification of viscoelastic properties suggests that elastography algorithms incorporating viscosity may be able to more accurately distinguish between pancreatic cancer and pancreatitis. STATEMENT OF SIGNIFICANCE:Understanding tumor-stroma crosstalk in pancreatic ductal adenocarcinoma (PDAC) is challenged by a lack of stroma-mimicking model systems. To design appropriate models, pancreatic tissue must be characterized with a method capable of evaluating in vitro models as well. Our indentation-based characterization tool quantified the distinct viscoelastic signatures of inflamed resections from pancreatitis, tumors from PDAC, and otherwise normal tissue to inform development of mechanically appropriate engineered tissues and scaffolds. We also made progress toward a 3D in vitro system that recapitulates mechanical properties of tumors. Our in vitro model of stromal cells in collagen and complementary characterization system can be used to investigate mechanisms of cancer-stroma crosstalk in PDAC and to propose and test innovative therapies.

Dataset Information

Viscoelastic Properties of ECM-Rich Embryonic Microenvironments.

Publications

Viscoelastic Properties of ECM-Rich Embryonic Microenvironments.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets