Ontology highlight
ABSTRACT:
SUBMITTER: Woo CH
PROVIDER: S-EPMC7499000 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Nature biomedical engineering 20200918 12
The control of viral outbreaks requires nucleic acid diagnostic tests that are sensitive, simple and fast. Here, we report a highly sensitive and specific one-pot assay for the fluorescence-based detection of RNA from pathogens. The assay, which can be performed within 30-50 min of incubation time and can reach a limit of detection of 0.1-attomolar RNA concentration, relies on a sustained isothermal reaction cascade producing an RNA aptamer that binds to a fluorogenic dye. The RNA aptamer is tra ...[more]