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Optoribogenetic control of regulatory RNA molecules.

ABSTRACT: Short regulatory RNA molecules underpin gene expression and govern cellular state and physiology. To establish an alternative layer of control over these processes, we generated chimeric regulatory RNAs that interact reversibly and light-dependently with the light-oxygen-voltage photoreceptor PAL. By harnessing this interaction, the function of micro RNAs (miRs) and short hairpin (sh) RNAs in mammalian cells can be regulated in a spatiotemporally precise manner. The underlying strategy is generic and can be adapted to near-arbitrary target sequences. Owing to full genetic encodability, it establishes optoribogenetic control of cell state and physiology. The method stands to facilitate the non-invasive, reversible and spatiotemporally resolved study of regulatory RNAs and protein function in cellular and organismal environments.

SUBMITTER: Pilsl S

PROVIDER: S-EPMC7518282 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Optoribogenetic control of regulatory RNA molecules.

Pilsl Sebastian S Morgan Charles C Choukeife Moujab M Möglich Andreas A Mayer Günter G

Nature communications 20200924 1

Short regulatory RNA molecules underpin gene expression and govern cellular state and physiology. To establish an alternative layer of control over these processes, we generated chimeric regulatory RNAs that interact reversibly and light-dependently with the light-oxygen-voltage photoreceptor PAL. By harnessing this interaction, the function of micro RNAs (miRs) and short hairpin (sh) RNAs in mammalian cells can be regulated in a spatiotemporally precise manner. The underlying strategy is generi ...[more]

PMID: 32973178

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