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Directional Structure Modification of Poplar Biomass-Inspired High Efficacy of Enzymatic Hydrolysis by Sequential Dilute Acid-Alkali Treatment.

ABSTRACT: A major challenge in converting lignocellulose to biofuel is overcoming the resistance of the biomass structure. Herein, sequential dilute acid-alkali/aqueous ammonia treatment was evaluated to enhance enzymatic hydrolysis of poplar biomass by removing hemicellulose first and then removing lignin with acid and base, respectively. The results show that glucose release in sequential dilute acid-alkali treatments (61.4-71.4 mg/g) was 7.3-24.8% higher than sequential dilute acid-aqueous ammonia treatments (57.2-61.8 mg/g) and 283.8-346.3% higher than control (16.0 mg/g), respectively. Dilute acid treatment removed most hemicellulose (84.9%) of the biomass, followed by alkaline treatment with 27.5% removal of lignin. Roughness, surface area, and micropore volume of the biomass were crucial for the enzymatic hydrolysis. Furthermore, the ultrastructure changes observed using crystallinity, Fourier transform infrared spectroscopy, thermogravimetric analysis, and pyrolysis gas chromatography/mass spectrometry support the effects of sequential dilute acid-alkali treatment. The results provide an efficient approach to facilitate a better enzymatic hydrolysis of the poplar samples.

SUBMITTER: Shi F

PROVIDER: S-EPMC7528282 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Directional Structure Modification of Poplar Biomass-Inspired High Efficacy of Enzymatic Hydrolysis by Sequential Dilute Acid-Alkali Treatment.

Shi Fuxi F Wang Yajun Y Davaritouchaee Maryam M Yao Yiqing Y Kang Kang K

ACS omega 20200921 38

A major challenge in converting lignocellulose to biofuel is overcoming the resistance of the biomass structure. Herein, sequential dilute acid-alkali/aqueous ammonia treatment was evaluated to enhance enzymatic hydrolysis of poplar biomass by removing hemicellulose first and then removing lignin with acid and base, respectively. The results show that glucose release in sequential dilute acid-alkali treatments (61.4-71.4 mg/g) was 7.3-24.8% higher than sequential dilute acid-aqueous ammonia trea ...[more]

PMID: 33015496

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Project description:BackgroundLiquid hot water (LHW) pretreatment has been considered as one of the most industrially viable and environment-friendly methods for facilitating the transformation of lignocelluloses into biofuels through biological conversion. However, lignin fragments in pretreatment hydrolysates are preferential to condense with each other and then deposit back onto cellulose surface under severe conditions. Particularly, lignin tends to relocate or redistribute under high-temperature LHW pretreatment conditions. The lignin residues on the cellulose surface would result in significant nonproductive binding of cellulolytic enzymes, and therefore negatively affect the enzymatic conversion (EC) of glucan in pretreated substrates. Although additives such as bovine serum albumin (BSA) and Tween series have been used to reduce nonproductive binding of enzymes through blocking the lignin, the high cost or non-biocompatibility of these additives limits their potential in industrial applications.ResultsHere, we firstly report that a soluble soy protein (SP) extracted from inexpensive defatted soy powder (DSP) showed excellent performance in promoting the EC of glucan in LHW-pretreated lignocellulosic substrates. The addition of the SP (80 mg/g glucan) could readily reduce the cellulase (Celluclast 1.5 L®) loading by 8 times from 96.7 to 12.1 mg protein/g glucan and achieve a glucan EC of 80% at a hydrolysis time of 72 h. With the same cellulase (Celluclast 1.5 L®) loading (24.2 mg protein/g glucan), the ECs of glucan in LHW-pretreated bamboo, eucalyptus, and Masson pine substrates increased from 57%, 54% and 45% (without SP) to 87%, 94% and 86% (with 80 mg SP/g glucan), respectively. Similar effects were also observed when Cellic CTec2, a newer-generation cellulase preparation, was used. Mechanistic studies indicated that the adsorption of soluble SP onto the surface of lignin residues could reduce the nonproductive binding of cellulolytic enzymes to lignin. The cost of the SP required for effective promotion would be equivalent to the cost of 2.9 mg cellulase (Celluclast 1.5 L®) protein (or 1.2 FPU/g glucan), if a proposed semi-simultaneous saccharification and fermentation (semi-SSF) model was used.ConclusionsNear-complete saccharification of glucan in LHW-pretreated lignocellulosic substrates could be achieved with the addition of the inexpensive and biocompatible SP additive extracted from DSP. This simple but remarkably effective technique could readily contribute to improving the economics of the cellulosic biorefinery industry.

Dataset Information

Directional Structure Modification of Poplar Biomass-Inspired High Efficacy of Enzymatic Hydrolysis by Sequential Dilute Acid-Alkali Treatment.

Publications

Directional Structure Modification of Poplar Biomass-Inspired High Efficacy of Enzymatic Hydrolysis by Sequential Dilute Acid-Alkali Treatment.

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