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Upregulation of CCT-3 Induces Breast Cancer Cell Proliferation Through miR-223 Competition and Wnt/?-Catenin Signaling Pathway Activation.

ABSTRACT: The clinical significance and the function of chaperonin-containing TCP1 complex 3 (CCT-3) in breast cancer remain unknown. In this study, we found that CCT-3 was markedly overexpressed in breast cancer tissues. Statistical analysis revealed a significant correlation of CCT-3 expression with advanced breast cancer clinical stage and poorer survival. Ablation of CCT-3 knocked down the proliferation and the tumorigenicity of breast cancer cells in vitro and in vivo. CCT-3 may regulate breast cancer cell proliferation through a ceRNA network between miR-223 and ?-catenin, thus affecting Wnt/?-catenin signaling pathway activation. We also validated that CCT-3 and ?-catenin are novel direct targets of tumor suppressor miR-223. Our results suggest that both mRNA and the protein levels of CCT-3 are potential diagnosis biomarkers and therapeutic targets for breast cancer.

SUBMITTER: Qu H

PROVIDER: S-EPMC7541898 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Upregulation of CCT-3 Induces Breast Cancer Cell Proliferation Through miR-223 Competition and Wnt/β-Catenin Signaling Pathway Activation.

Qu Hongbo H Zhu Fang F Dong Huaying H Hu Xiongqiang X Han Mingli M

Frontiers in oncology 20200924

The clinical significance and the function of chaperonin-containing TCP1 complex 3 (CCT-3) in breast cancer remain unknown. In this study, we found that CCT-3 was markedly overexpressed in breast cancer tissues. Statistical analysis revealed a significant correlation of CCT-3 expression with advanced breast cancer clinical stage and poorer survival. Ablation of CCT-3 knocked down the proliferation and the tumorigenicity of breast cancer cells <i>in vitro</i> and <i>in vivo</i>. CCT-3 may regulat ...[more]

PMID: 33072568

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