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JCAD expression and localization in human blood endothelial cells.

ABSTRACT: Background:Junctional Cadherin 5 Associated (JCAD) is an endothelial, cell-cell junction protein, and its expression is associated with cardiovascular diseases including atherosclerosis and hypertension. However, to date, there are few studies confirming JCAD expression and precise localization in human tissues by immunohistochemical staining. Methods:JCAD expression and localization was assessed in four human submandibular gland (SMG) specimens by immunohistochemical staining. One specimen of SMG with sialoadenitis was accompanied by severe inflammation and fibrosis, while the other was largely normal. Other two SMGs were accompanied by severe fibrosis because of irradiation. Results:Immunohistochemical analysis of human SMGs revealed JCAD localization at the blood endothelial cell-cell junctions. JCAD expression was more evident in microvessels and arteries in areas affected by inflammation. Conclusions:The localization of JCAD at endothelial cell-cell junctions was confirmed in human tissues. JCAD expression may be affected by pathological conditions.

SUBMITTER: Shigeoka M

PROVIDER: S-EPMC7550929 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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JCAD expression and localization in human blood endothelial cells.

Shigeoka Manabu M Arimoto Satomi S Akashi Masaya M

Heliyon 20201008 10

<h4>Background</h4>Junctional Cadherin 5 Associated (JCAD) is an endothelial, cell-cell junction protein, and its expression is associated with cardiovascular diseases including atherosclerosis and hypertension. However, to date, there are few studies confirming JCAD expression and precise localization in human tissues by immunohistochemical staining.<h4>Methods</h4>JCAD expression and localization was assessed in four human submandibular gland (SMG) specimens by immunohistochemical staining. On ...[more]

PMID: 33083606

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Project description:RNA may be released from vascular cells including endothelial cells in the event of injury and in vascular disease. Extracellular RNAs have been recognized as novel procoagulant and permeability-increasing factors. Extracellular RNA may function as inflammatory host alarm signals that serve to amplify the defense mechanism, but it may provide important links to thrombus formation. Extracellular RNA is degraded by RNase. We propose that RNase and its inhibitor RNase inhibitor (RI) act as modulators of homeostasis in the vasculature to control the functions of extracellular RNA. We aimed to investigate the expression and localization of RNase 1 and RI in cells that contact blood, such as platelets, mononuclear cells, polymorphonuclear cells, and red blood cells. RNase 1 and RI expression and localization in blood cells were compared with those in the human umbilical vein endothelial cell line, EAhy926. Additionally, we further investigated the effect of thrombin on the expression of RNase 1 and RI in platelets. We used an RNase activity assay, reverse transcription-polymerase chain reaction, western blot, immunocytochemistry, transmission electron microscopy, and immunoelectron microscopy (pre- and post-embedding methods). RNase activity in the supernatant from EAhy926 cells was 50 times than in blood cells (after 60 min). RNase 1 mRNA and protein expression in EAhy926 cells was highest among the cells examined. However, RI mRNA and protein expression was similar in most cell types examined. Furthermore, we observed that RNase 1 and von Willebrand factor were partially colocalized in EAhy926 cells and platelets. In conclusion, we propose that high RNase activity is ordinarily released from endothelial cells to support anticoagulation in the vasculature. On the other hand, platelets and leukocytes within thrombi at sites of vascular injury show very low RNase activity, which may support hemostatic thrombus formation. However, activated platelets and leukocytes may accelerate pathologic thrombus formation.

Dataset Information

JCAD expression and localization in human blood endothelial cells.

Publications

JCAD expression and localization in human blood endothelial cells.

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