Dataset Information

Early Embryonic Expression of AP-2? Is Critical for Cardiovascular Development.

ABSTRACT: Congenital cardiovascular malformation is a common birth defect incorporating abnormalities of the outflow tract and aortic arch arteries, and mice deficient in the transcription factor AP-2? (Tcfap2a) present with complex defects affecting these structures. AP-2? is expressed in the pharyngeal surface ectoderm and neural crest at mid-embryogenesis in the mouse, but the precise tissue compartment in which AP-2? is required for cardiovascular development has not been identified. In this study we describe the fully penetrant AP-2? deficient cardiovascular phenotype on a C57Bl/6J genetic background and show that this is associated with increased apoptosis in the pharyngeal ectoderm. Neural crest cell migration into the pharyngeal arches was not affected. Cre-expressing transgenic mice were used in conjunction with an AP-2? conditional allele to examine the effect of deleting AP-2? from the pharyngeal surface ectoderm and the neural crest, either individually or in combination, as well as the second heart field. This, surprisingly, was unable to fully recapitulate the global AP-2? deficient cardiovascular phenotype. The outflow tract and arch artery phenotype was, however, recapitulated through early embryonic Cre-mediated recombination. These findings indicate that AP-2? has a complex influence on cardiovascular development either being required very early in embryogenesis and/or having a redundant function in many tissue layers.

SUBMITTER: Johnson AL

PROVIDER: S-EPMC7570199 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Early Embryonic Expression of AP-2α Is Critical for Cardiovascular Development.

Johnson Amy-Leigh AL Schneider Jürgen E JE Mohun Timothy J TJ Williams Trevor T Bhattacharya Shoumo S Henderson Deborah J DJ Phillips Helen M HM Bamforth Simon D SD

Journal of cardiovascular development and disease 20200723 3

Congenital cardiovascular malformation is a common birth defect incorporating abnormalities of the outflow tract and aortic arch arteries, and mice deficient in the transcription factor AP-2α (Tcfap2a) present with complex defects affecting these structures. AP-2α is expressed in the pharyngeal surface ectoderm and neural crest at mid-embryogenesis in the mouse, but the precise tissue compartment in which AP-2α is required for cardiovascular development has not been identifi ...[more]

PMID: 32717817

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Dataset Information

Early Embryonic Expression of AP-2? Is Critical for Cardiovascular Development.

Publications

Early Embryonic Expression of <i>AP-2α</i> Is Critical for Cardiovascular Development.

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