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Protocol for Imaging of Mitoflashes in Live Cardiomyocytes.

ABSTRACT: We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function in situ. The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protocol, please refer to Gong et al. (2014) and Gong et al. (2015).

SUBMITTER: Li A

PROVIDER: S-EPMC7580095 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Protocol for Imaging of Mitoflashes in Live Cardiomyocytes.

Li Anqi A Qin Yuan Y Gao Meng M Jiang Wenting W Liu Bilin B Tian Xiangang X Gong Guohua G

STAR protocols 20200903 2

We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function <i>in situ</i>. The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protoco ...[more]

PMID: 33111128

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