Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
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ABSTRACT:
SUBMITTER: Hor P
PROVIDER: S-EPMC7580194 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
Hor Pooja P Ichida Justin K JK Borok Zea Z Ryan Amy L AL
STAR protocols 20200724 2
Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to ...[more]
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