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A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression.

ABSTRACT: A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

SUBMITTER: Imoto T

PROVIDER: S-EPMC7596873 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression.

Imoto Takuma T Minoshima Masafumi M Yokoyama Tatsushi T Emery Ben P BP Bull Steven D SD Bito Haruhiko H Kikuchi Kazuya K

ACS central science 20200831 10

A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription com ...[more]

PMID: 33145417

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