Inducible depletion of PI(4,5)P₂ by the synthetic iDePP system in Arabidopsis.

ABSTRACT: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂) is a low-abundance membrane lipid essential for plasma membrane function^1,2. In plants, mutations in phosphatidylinositol 4-phosphate (PI4P) 5-kinases (PIP5K) suggest that PI(4,5)P₂ production is involved in development, immunity and reproduction^3-5. However, phospholipid synthesis is highly intricate⁶. It is thus likely that steady-state depletion of PI(4,5)P₂ triggers confounding indirect effects. Furthermore, inducible tools available in plants allow PI(4,5)P₂ to increase^7-9 but not decrease, and no PIP5K inhibitors are available. Here, we introduce iDePP (inducible depletion of PI(4,5)P₂ in plants), a system for the inducible and tunable d

SUBMITTER: Doumane M 

PROVIDER: S-EPMC7610831 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature