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Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

ABSTRACT: The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo.

SUBMITTER: Worrall JA

PROVIDER: S-EPMC7611026 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure.

De Chiara Matteo M Hood Derek D Muzzi Alessandro A Pickard Derek J DJ Perkins Tim T Pizza Mariagrazia M Dougan Gordon G Rappuoli Rino R Moxon E Richard ER Soriani Marco M Donati Claudio C

Proceedings of the National Academy of Sciences of the United States of America 20140325 14

One of the main hurdles for the development of an effective and broadly protective vaccine against nonencapsulated isolates of Haemophilus influenzae (NTHi) lies in the genetic diversity of the species, which renders extremely difficult the identification of cross-protective candidate antigens. To assess whether a population structure of NTHi could be defined, we performed genome sequencing of a collection of diverse clinical isolates representative of both carriage and disease and of the divers ...[more]

PMID: 24706866

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Global analysis of Escherichia coli RNA degradosome function using DNA microarrays.

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Global analysis of Escherichia coli RNA degradosome function

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Global analysis of Escherichia coli RNA degradosome function

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