Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

ABSTRACT: Despite its size and rigidity, the cell nucleus can be moved or reorganized by cytoskeletal filaments under various conditions (for example, during viral infection)^1-11. Moreover, whereas chromatin organizes into non-random domains¹², extensive heterogeneity at the single-cell level¹³ means that precisely how and why nuclei reorganize remains an area of intense investigation. Here we describe convolutional neural network-based automated cell classification and analysis pipelines, which revealed the extent to which human cytomegalovirus generates nuclear polarity through a virus-assembled microtubule-organizing centre. Acetylation of tubulin enables microtubules emanating from this centre to rotate the nucleus by engaging cytoplasmically exposed dynein-bindi

SUBMITTER: Procter DJ 

PROVIDER: S-EPMC7644666 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature