Ontology highlight
ABSTRACT:
SUBMITTER: de Wet TJ
PROVIDER: S-EPMC7647400 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
de Wet Timothy J TJ Winkler Kristy R KR Mhlanga Musa M Mizrahi Valerie V Warner Digby F DF
eLife 20201106
<i>Mycobacterium tuberculosis</i> possesses a large number of genes of unknown or predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interference and image-based analyses of morphological features and sub-cellular chromosomal localizations in the related non-pathogen, <i>M. smegmatis</i>. Applying automated imaging and analysis to 263 ...[more]