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The smfBox is an open-source platform for single-molecule FRET.


ABSTRACT: Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

SUBMITTER: Ambrose B 

PROVIDER: S-EPMC7648814 | biostudies-literature |

REPOSITORIES: biostudies-literature

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