Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe S
PROVIDER: S-EPMC7658699 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Watanabe Sadanori S Meitinger Franz F Shiau Andrew K AK Oegema Karen K Desai Arshad A
The Journal of cell biology 20201201 12
Centrosomes, composed of centrioles that recruit a pericentriolar material (PCM) matrix assembled from PCNT and CDK5RAP2, catalyze mitotic spindle assembly. Here, we inhibit centriole formation and/or remove PCNT-CDK5RAP2 in RPE1 cells to address their relative contributions to spindle formation. While CDK5RAP2 and PCNT are normally dispensable for spindle formation, they become essential when centrioles are absent. Acentriolar spindle assembly is accompanied by the formation of foci containing ...[more]