Unknown

Dataset Information

0

Viral rhodopsins 1 are an unique family of light-gated cation channels.


ABSTRACT: Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na+/K+-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca2+ ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4?Å resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca2+ permeability.

SUBMITTER: Zabelskii D 

PROVIDER: S-EPMC7659345 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Viral rhodopsins 1 are an unique family of light-gated cation channels.

Zabelskii Dmitrii D   Alekseev Alexey A   Kovalev Kirill K   Rankovic Vladan V   Balandin Taras T   Soloviov Dmytro D   Bratanov Dmitry D   Savelyeva Ekaterina E   Podolyak Elizaveta E   Volkov Dmytro D   Vaganova Svetlana S   Astashkin Roman R   Chizhov Igor I   Yutin Natalia N   Rulev Maksim M   Popov Alexander A   Eria-Oliveira Ana-Sofia AS   Rokitskaya Tatiana T   Mager Thomas T   Antonenko Yuri Y   Rosselli Riccardo R   Armeev Grigoriy G   Shaitan Konstantin K   Vivaudou Michel M   Büldt Georg G   Rogachev Andrey A   Rodriguez-Valera Francisco F   Kirpichnikov Mikhail M   Moser Tobias T   Offenhäusser Andreas A   Willbold Dieter D   Koonin Eugene E   Bamberg Ernst E   Gordeliy Valentin V  

Nature communications 20201111 1


Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective  ...[more]

Similar Datasets

| S-EPMC4764398 | biostudies-literature
| S-EPMC6821725 | biostudies-literature
| S-EPMC4160518 | biostudies-literature
| S-EPMC3362136 | biostudies-literature
| S-EPMC4332693 | biostudies-literature
| S-EPMC283525 | biostudies-literature
| S-EPMC1574977 | biostudies-literature
| S-EPMC7541481 | biostudies-literature
| S-EPMC4702976 | biostudies-literature
| S-EPMC3431587 | biostudies-literature