Ontology highlight
ABSTRACT:
SUBMITTER: Bury L
PROVIDER: S-EPMC7679138 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Bury Leah L Moodie Brittania B Ly Jimmy J McKay Liliana S LS Miga Karen Hh KH Cheeseman Iain M IM
eLife 20201111
Although originally thought to be silent chromosomal regions, centromeres are instead actively transcribed. However, the behavior and contributions of centromere-derived RNAs have remained unclear. Here, we used single-molecule fluorescence in-situ hybridization (smFISH) to detect alpha-satellite RNA transcripts in intact human cells. We find that alpha-satellite RNA-smFISH foci levels vary across cell lines and over the cell cycle, but do not remain associated with centromeres, displaying local ...[more]