Dataset Information

Identification of alpha-satellite RNA interacting proteins

ABSTRACT: We set out to identify proteins that bind alpha-satellite ncRNAs in HCT-116 and SW-480 colorectal cancer human cell lines. We performed RNA pulldown followed by mass spectrometry identification of the associated proteins. Among the identified proteins, we observed a statistical overrepresenttion of some ontology ssociated to mitotic progression in HCT-116 cells, but not in SW-480 cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Rodrigo Cáceres

LAB HEAD: Luis A. Herrera

PROVIDER: PXD024582 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Proteasome inhibition alters mitotic progression through the upregulation of centromeric α-Satellite RNAs.

Cáceres-Gutiérrez Rodrigo E RE Andonegui Marco A MA Oliva-Rico Diego A DA González-Barrios Rodrigo R Luna Fernando F Arriaga-Canon Cristian C López-Saavedra Alejandro A Prada Diddier D Castro Clementina C Parmentier Laurent L Díaz-Chávez José J Alfaro-Mora Yair Y Navarro-Delgado Erick I EI Fabian-Morales Eunice E Tran Bao B Shetty Jyoti J Zhao Yongmei Y Alcaraz Nicolas N De la Rosa Carlos C Reyes José L JL Hédouin Sabrine S Hubé Florent F Francastel Claire C Herrera Luis A LA

The FEBS journal 20211118 7

Cell cycle progression requires control of the abundance of several proteins and RNAs over space and time to properly transit from one phase to the next and to ensure faithful genomic inheritance in daughter cells. The proteasome, the main protein degradation system of the cell, facilitates the establishment of a proteome specific to each phase of the cell cycle. Its activity also strongly influences transcription. Here, we detected the upregulation of repetitive RNAs upon proteasome inhibition ...[more]

PMID: 34739170

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