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Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-?1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

ABSTRACT: The effects of mesenchymal stem cells (MSCs) on different types of diseases are controversial, and the inner mechanisms remain unknown, which retards the utilization of MSCs in disease therapy. In this study, we aimed to elucidate the mechanisms of MSCs-extracellular vesicles (EVs) carrying transforming growth factor-beta 1 (TGF-?1) in M2 polarization in mouse macrophages via the microRNA-132 (miR-132)/E3 ubiquitin ligase myc binding protein 2 (Mycbp2)/tuberous sclerosis complex 2 (TSC2) axis. Mouse MSCs were isolated for adipogenic and osteogenic induction, followed by co-culture with mouse macrophages RAW264.7. Besides, mouse macrophages RAW264.7 were co-cultured with MSCs-EVs in vitro, where the proportion of macrophages and inflammation were detected by flow cytometry and ELISA. The experimental data revealed that MSCs-EVs promoted M2 polarization of macrophages, and elevated interleukin (IL)-10 expression and inhibited levels of IL-1?, tumour necrosis factor (TNF)-? and IL-6. MSC-EV-treated macrophages RAW264.7 increased TGF-?1 expression, thus elevating miR-132 expression. MiR-132 directly bound to Mycbp2, as confirmed by luciferase activity assay. Meanwhile, E3 ubiquitin ligase Mycbp2 could ubiquitinate TSC2 protein. Furthermore, silencing TGF-?1 inhibited M2 polarization of MSC-EV-treated macrophages. Taken conjointly, this study provides evidence reporting that MSC-secreted EVs carry TGF-?1 to promote M2 polarization of macrophages via modulation of the miR-132/Mycbp2/TSC2 axis.

SUBMITTER: Wang Y

PROVIDER: S-EPMC7686990 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

Wang Yongqi Y Han Biao B Wang Yingbin Y Wang Chunai C Zhang Hong H Xue Jianjun J Wang Xiaoqing X Niu Tingting T Niu Zhen Z Chen Yuhe Y

Journal of cellular and molecular medicine 20200923 21

The effects of mesenchymal stem cells (MSCs) on different types of diseases are controversial, and the inner mechanisms remain unknown, which retards the utilization of MSCs in disease therapy. In this study, we aimed to elucidate the mechanisms of MSCs-extracellular vesicles (EVs) carrying transforming growth factor-beta 1 (TGF-β1) in M2 polarization in mouse macrophages via the microRNA-132 (miR-132)/E3 ubiquitin ligase myc binding protein 2 (Mycbp2)/tuberous sclerosis complex 2 (TSC2) axis. M ...[more]

PMID: 32965772

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Project description:BACKGROUND:An uncontrolled inflammatory response is a critical pathophysiological feature of sepsis. Mesenchymal stem cells (MSCs) induce macrophage phenotype polarization and reduce inflammation in sepsis. MSC-secreted transforming growth factor beta (TGF-?) participated in the immune modulatory function of MSCs. However, the underlying mechanism of MSC-secreted TGF-? was not fully elucidated in regulation macrophage M2-like polarization. METHODS:The paracrine effects of MSCs on macrophage polarization were studied using a co-culture protocol with LPS-stimulated RAW264.7 cells/mouse peritoneal macrophages and MSCs. The effect of TGF-? in the co-culture system was blocked by the TGF-? receptor inhibitor. To determine the role of MSC-secreted TGF-?, we used recombinant TGF-? to culture with LPS-stimulated RAW264.7 cells. In addition, we employed antibody microarray analysis to determine the mechanisms of MSC secreted TGF-? on LPS-stimulated RAW264.7 cell/mouse peritoneal macrophage M2-like polarization. Furthermore, we used an Akt inhibitor and a FoxO1 inhibitor to inhibit the Akt/FoxO1 pathway. The nuclear translocation of FoxO1 was detected by Western blot. RESULTS:MSCs induced LPS-stimulated RAW264.7 cell/mouse peritoneal macrophage polarization towards the M2-like phenotype and significantly reduced pro-inflammatory cytokine levels via paracrine, which was inhibited by TGF-? receptor inhibitor. Furthermore, we found that MSC-secreted TGF-? enhanced the macrophage phagocytic ability. The antibody microarray analysis and Western blot verified that TGF-? treatment activated the Akt/FoxO1 pathway in LPS-stimulated macrophages, TGF-?-induced FoxO1 nuclear translocation and obviously expressed in the cytoplasm, the effects of TGF-? regulatory effects on LPS-stimulated macrophage were inhibited by pre-treatment with Akt inhibitor and FoxO1 inhibitor. CONCLUSIONS:TGF-? secreted by MSCs could skew LPS-stimulated macrophage polarization towards the M2-like phenotype, reduce inflammatory reactions, and improve the phagocytic ability via the Akt/FoxO1 pathway, providing potential therapeutic strategies for sepsis.

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Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-?1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

Publications

Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

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