Dataset Information

Polyherbal formula (ASILACT®) induces Milk production in lactating rats through Upregulation of ?-Lactalbumin and aquaporin expression.

ABSTRACT:

Background

Polyherbal formula (PHF) contains extract of Sauropus androgynous (L.) Merr., Trigonella foenum-graceum L., and Moringa oleifera Lam. considered to induce galactagogue activity. This research aimed to evaluate the galactagogue activity of PHF and its effects on ?-lactalbumin (LALBA) as well as aquaporin (AQP) gene expression at messenger ribonucleic acid (mRNA) levels in mammary glands of lactating rats.

Methods

Thirty lactating Wistar rats were randomly divided into five groups (n?=?6), each has 7 pups. Group I was treated orally with distilled water as negative control. Groups II, III, and IV were orally administered with PHF at 26.25, 52.5 and 105?mg/kg/day, respectively. Group V was treated with domperidone 2.7?mg/kg/day, orally as positive control. The treatment was performed at third day until fifteenth day of parturition. The observed parameters include the galactagogue activity indicating by milk yield of lactating rats, the pup weight changes and lactating rats body weight changes during lactating period, mRNA expression of LALBA and AQP using quantitative Real Time Polymerase Chain Reaction (qRT-PCR) and histopathological analysis of mammary glands at the end of treatment period.

Result

The result showed that the PHF groups (52.5 and 105?mg/kg/day) and domperidone were significantly increased milk production of lactating rats (p?ConclusionThe study suggested that PHF has potentially to induce galactagogue activity on lactating period through upregulation of LALBA and AQP genes at the mRNA level.

SUBMITTER: Mustofa

PROVIDER: S-EPMC7690098 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Publications

Polyherbal formula (ASILACT®) induces Milk production in lactating rats through Upregulation of α-Lactalbumin and aquaporin expression.

Mustofa Yuliani Fara Silvia FS Purwono Setyo S Sadewa Ahmad Hamim AH Damayanti Ema E Heriyanto Didik Setyo DS

BMC complementary medicine and therapies 20201126 1

<h4>Background</h4>Polyherbal formula (PHF) contains extract of Sauropus androgynous (L.) Merr., Trigonella foenum-graceum L., and Moringa oleifera Lam. considered to induce galactagogue activity. This research aimed to evaluate the galactagogue activity of PHF and its effects on α-lactalbumin (LALBA) as well as aquaporin (AQP) gene expression at messenger ribonucleic acid (mRNA) levels in mammary glands of lactating rats.<h4>Methods</h4>Thirty lactating Wistar rats were randomly divided into fi ...[more]

PMID: 33243201

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