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Hetero-Diels-Alder Cycloaddition with RAFT Polymers as Bioconjugation Platform.

ABSTRACT: We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels-Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains.

SUBMITTER: Beloqui A

PROVIDER: S-EPMC7693046 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Hetero-Diels-Alder Cycloaddition with RAFT Polymers as Bioconjugation Platform.

Beloqui Ana A Mane Shivshankar R SR Langer Marcel M Glassner Mathias M Bauer Dennis M DM Fruk Ljiljana L Barner-Kowollik Christopher C Delaittre Guillaume G

Angewandte Chemie (International ed. in English) 20200902 45

We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels-Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlle ...[more]

PMID: 32729643

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