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Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes.

ABSTRACT: There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines.

SUBMITTER: Jain PK

PROVIDER: S-EPMC7709491 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes.

Jain Piyush K PK Lo Justin H JH Rananaware Santosh S Downing Marco M Panda Apekshya A Tai Michelle M Raghavan Srivatsan S Fleming Heather E HE Bhatia Sangeeta N SN

Nanoscale 20191031 44

There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/C ...[more]

PMID: 31670340

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