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Interaction of amyloid beta with humanin and acetylcholinesterase is modulated by ATP.


ABSTRACT: Humanin (HN) is known to bind amyloid beta (A?)-inducing cytoprotective effects, while binding of acetylcholinesterase (AChE) to A? increases its aggregation and cytotoxicity. Previously, we showed that binding of HN to A? blocks aggregation induced by AChE and that HN decreases but does not abolish A?-AChE interactions in A549 cell media. Here, we set out to shed light on factors that modulate the interactions of A? with HN and AChE. We found that binding of either HN or AChE to A? is not affected by heparan sulfate, while ATP, thought to reduce misfolding of A?, weakened interactions between AChE and A? but strengthened those between A? and HN. Using media from either A549 or H1299 lung cancer cells, we observed that more HN was bound to A? upon addition of ATP, while levels of AChE in a complex with A? were decreased by ATP addition to A549 cell media. Exogenous addition of ATP to either A549 or H1299 cell media increased interactions of endogenous HN with A? to a comparable extent despite differences in AChE expression in the two cell lines, and this was correlated with decreased binding of exogenously added HN to A?. Treatment with exogenous ATP had no effect on cell viability under all conditions examined. Exogenously added ATP did not affect viability of cells treated with AChE-immunodepleted media, and there was no apparent protection against the cytotoxicity resulting from immunodepletion of HN. Moreover, exogenously added ATP had no effect on the relative abundance of oligomer versus total A? in either cell line.

SUBMITTER: Atali S 

PROVIDER: S-EPMC7714071 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Interaction of amyloid beta with humanin and acetylcholinesterase is modulated by ATP.

Atali Sarah S   Dorandish Sadaf S   Devos Jonathan J   Williams Asana A   Price Deanna D   Taylor Jaylen J   Guthrie Jeffrey J   Heyl Deborah D   Evans Hedeel Guy HG  

FEBS open bio 20201118 12


Humanin (HN) is known to bind amyloid beta (Aβ)-inducing cytoprotective effects, while binding of acetylcholinesterase (AChE) to Aβ increases its aggregation and cytotoxicity. Previously, we showed that binding of HN to Aβ blocks aggregation induced by AChE and that HN decreases but does not abolish Aβ-AChE interactions in A549 cell media. Here, we set out to shed light on factors that modulate the interactions of Aβ with HN and AChE. We found that binding of either HN or AChE to Aβ is not affec  ...[more]

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