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Cryo-EM structure of Bacillus subtilis RNA Polymerase in complex with HelD

ABSTRACT:

SUBMITTER: Gokhan Tolun

PROVIDER: EMPIAR-11052 | biostudies-other |

REPOSITORIES: biostudies-other

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Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD.

Newing Timothy P TP Oakley Aaron J AJ Miller Michael M Dawson Catherine J CJ Brown Simon H J SHJ Bouwer James C JC Tolun Gökhan G Lewis Peter J PJ

Nature communications 20201218 1

In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled complexes, but it was not known how it performed this function. Here, using single particle cryo-electron microscopy, we have determined the structures of Bacillus subtilis RNA polymerase (RNAP) elongation and H ...[more]

PMID: 33339820

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