Ontology highlight
ABSTRACT:
SUBMITTER: Mehlich A
PROVIDER: S-EPMC7750197 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Frontiers in chemistry 20201207
<i>De-novo</i> designed proteins have received wide interest as potential platforms for nano-engineering and biomedicine. While much work is being done in the design of thermodynamically stable proteins, the folding process of artificially designed proteins is not well-studied. Here we used single-molecule force spectroscopy by optical tweezers to study the folding of ROSS, a <i>de-novo</i> designed 2x2 Rossmann fold. We measured a barrier crossing time in the millisecond range, much slower than ...[more]