Ontology highlight
ABSTRACT:
SUBMITTER: Baumschabl M
PROVIDER: S-EPMC7754189 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Baumschabl Michael M Prielhofer Roland R Mattanovich Diethard D Steiger Matthias G MG
ACS synthetic biology 20201112 12
For metabolic engineering approaches, fast and reliable tools are required to precisely manipulate the expression of target genes. dCas9 can be fused <i>via</i> RNA scaffolds to trans-activator domains and thus regulate the gene expression when targeted to the promoter region of a gene. In this work we show that this strategy can be successfully implemented for the methylotrophic yeast <i>Pichia pastoris</i>. It is shown that the thiamine repressible promoter of <i>THI11</i> can be activated und ...[more]