De Novo Generation of Murine and Human MADR Recipient Cell Lines for Locus-Specific, Stable Integration of Transgenic Elements.
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ABSTRACT:
SUBMITTER: Ayala-Sarmiento AE
PROVIDER: S-EPMC7757409 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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<i>De Novo</i> Generation of Murine and Human MADR Recipient Cell Lines for Locus-Specific, Stable Integration of Transgenic Elements.
Ayala-Sarmiento Alberto E AE Kobritz Naomi N Breunig Joshua J JJ
STAR protocols 20201125 3
Mosaic analysis by dual recombinase-mediated cassette exchange (MADR) is a technology that allows stable and locus-specific integration of transgenic elements into recipient cells carrying loxP and FRT sites. Nevertheless, most cell lines lack these recombination-specific sites. This protocol describes a method to introduce the minimum requirements into cells, leading to the generation of <i>de novo</i> primary MADR recipient cells or MADR "Proxy" cells. These cell lines allow the combinatorial ...[more]
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