Dataset Information

Plasma BCAA concentrations during exercise of varied intensities in young healthy men-the impact of endurance training.

ABSTRACT:

Background

Branched-chain amino acids (BCAA) i.e., leucine (Leu), isoleucine (Ile) and valine (Val) are important amino acids, which metabolism play a role in maintaining system energy homeostasis at rest and during exercise. As recently shown lowering of circulating BCAA level improves insulin sensitivity and cardiac metabolic health. However, little is known concerning the impact of a single bout of incremental exercise and physical training on the changes in blood BCAA. The present study aimed to determine the impact of a gradually increasing exercise intensity-up to maximal oxygen uptake (VO_2max) on the changes of the plasma BCAA [?BCAA]_pl, before and after 5-weeks of moderate-intensity endurance training (ET).

Methods

Ten healthy young, untrained men performed an incremental cycling exercise test up to exhaustion to reach VO_2max, before and after ET.

Results

We have found that exercise of low-to-moderate intensity (up to ?50% of VO_2max lasting about 12 min) had no significant effect on the [?BCAA]_pl, however the exercise of higher intensity (above 70% of VO_2max lasting about 10 min) resulted in a pronounced decrease (p < 0.05) in [?BCAA]_pl. The lowering of plasma BCAA when performing exercise of higher intensity was preceded by a significant increase in plasma lactate concentration, showing that a significant attenuation of BCAA during incremental exercise coincides with exercise-induced acceleration of glycogen utilization. In addition, endurance training, which significantly increased power generating capabilities at VO_2max (p = 0.004) had no significant impact on the changes of [?BCAA]_pl during this incremental exercise.

Conclusion

We have concluded that an exercise of moderate intensity of relatively short duration generally has no effect on the [?BCAA]_pl in young, healthy men, whereas significant decrease in [?BCAA]_pl occurs when performing exercise in heavy-intensity domain. The impact of exercise intensity on the plasma BCAA concentration seems to be especially important for patients with cardiometabolic risk undertaken cardiac rehabilitation or recreational activity.

SUBMITTER: Gawedzka A

PROVIDER: S-EPMC7759138 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Plasma BCAA concentrations during exercise of varied intensities in young healthy men-the impact of endurance training.

Gawedzka Anna A Grandys Marcin M Duda Krzysztof K Zapart-Bukowska Justyna J Zoladz Jerzy A JA Majerczak Joanna J

PeerJ 20201221

<h4>Background</h4>Branched-chain amino acids (BCAA) i.e., leucine (Leu), isoleucine (Ile) and valine (Val) are important amino acids, which metabolism play a role in maintaining system energy homeostasis at rest and during exercise. As recently shown lowering of circulating BCAA level improves insulin sensitivity and cardiac metabolic health. However, little is known concerning the impact of a single bout of incremental exercise and physical training on the changes in blood BCAA. The present st ...[more]

PMID: 33391874

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Project description:Introduction: Endurance exercise alters whole-body as well as skeletal muscle metabolism and physiology, leading to improvements in performance and health. However, biological mechanisms underlying the body's adaptations to different endurance exercise protocols are not entirely understood. Methods: We applied a multi-platform metabolomics approach to identify urinary metabolites and associated metabolic pathways that distinguish the acute metabolic response to two endurance exercise interventions at distinct intensities. In our randomized crossover study, 16 healthy, young, and physically active men performed 30 min of continuous moderate exercise (CME) and continuous vigorous exercise (CVE). Urine was collected during three post-exercise sampling phases (U01/U02/U03: until 45/105/195 min post-exercise), providing detailed temporal information on the response of the urinary metabolome to CME and CVE. Also, fasting spot urine samples were collected pre-exercise (U00) and on the following day (U04). While untargeted two-dimensional gas chromatography-mass spectrometry (GC×GC-MS) led to the detection of 608 spectral features, 44 metabolites were identified and quantified by targeted nuclear magnetic resonance (NMR) spectroscopy or liquid chromatography-mass spectrometry (LC-MS). Results: 104 urinary metabolites showed at least one significant difference for selected comparisons of sampling time points within or between exercise trials as well as a relevant median fold change >1.5 or <0. 6¯ (NMR, LC-MS) or >2.0 or <0.5 (GC×GC-MS), being classified as either exercise-responsive or intensity-dependent. Our findings indicate that CVE induced more profound alterations in the urinary metabolome than CME, especially at U01, returning to baseline within 24 h after U00. Most differences between exercise trials are likely to reflect higher energy requirements during CVE, as demonstrated by greater shifts in metabolites related to glycolysis (e.g., lactate, pyruvate), tricarboxylic acid cycle (e.g., cis-aconitate, malate), purine nucleotide breakdown (e.g., hypoxanthine), and amino acid mobilization (e.g., alanine) or degradation (e.g., 4-hydroxyphenylacetate). Discussion: To conclude, this study provided first evidence of specific urinary metabolites as potential metabolic markers of endurance exercise intensity. Future studies are needed to validate our results and to examine whether acute metabolite changes in urine might also be partly reflective of mechanisms underlying the health- or performance-enhancing effects of endurance exercise, particularly if performed at high intensities.

Dataset Information

Plasma BCAA concentrations during exercise of varied intensities in young healthy men-the impact of endurance training.

Background

Methods

Results

Conclusion

Publications

Plasma BCAA concentrations during exercise of varied intensities in young healthy men-the impact of endurance training.

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