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Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.

ABSTRACT: Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations.

SUBMITTER: Chen H

PROVIDER: S-EPMC7775643 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.

Chen Haiqi H Liu Sophia S Padula Samuel S Lesman Daniel D Griswold Kettner K Lin Allen A Zhao Tongtong T Marshall Jamie L JL Chen Fei F

Nature biotechnology 20191216 2

Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations. ...[more]

PMID: 31844291

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