Ontology highlight
ABSTRACT:
SUBMITTER: Chen H
PROVIDER: S-EPMC7775643 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Chen Haiqi H Liu Sophia S Padula Samuel S Lesman Daniel D Griswold Kettner K Lin Allen A Zhao Tongtong T Marshall Jamie L JL Chen Fei F
Nature biotechnology 20191216 2
Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations. ...[more]