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The mitogenome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae).

ABSTRACT: In this study, the mitochondrial genome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae) was sequenced and annotated. The mitogenome of M. margheritae is 15376 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The A + T content in the mitogenome was 79%. Most of PCGs started with ATN and stopped with TAA while ATP8 and ND5 started with TTG, COX2, ND1 stopped with incomplete T, Cytb stopped with TAG. We further constructed a Bayesian phylogenetic tree among M. margheritae, M. albovittata and other Cicadellidae species. The constructed phylogenetic tree suggests the close evolutionary relationship between M. margheritae and M. albovittata.

SUBMITTER: He H

PROVIDER: S-EPMC7782090 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background:Coelidiinae, a relatively large subfamily within the family Cicadellidae, includes 129 genera and ?1,300 species distributed worldwide. However, the mitogenomes of only two species (Olidiana sp. and Taharana fasciana) in the subfamily Coelidiinae have been assembled. Here, we report the first complete mitogenome assembly of the genus Olidiana. Methods:Specimens were collected from Wenxian County (Gansu Province, China) and identified on the basis of their morphology. Mitogenomes were sequenced by next-generation sequencing, following which an NGS template was generated, and this was confirmed using polymerase chain reaction and Sanger sequencing. Phylogenic trees were constructed using maximum likelihood and Bayesian analyses. Results:The mitogenome of O. ritcheriina was 15,166 bp long, with an A + T content of 78.0%. Compared with the mitogenome of other Cicadellidae sp., the gene order, gene content, gene size, base composition, and codon usage of protein-coding genes (PCGs) in O. ritcheriina were highly conserved. The standard start codon of all PCGs was ATN and stop codon was TAA or TAG; COII, COIII, and ND4L ended with a single T. All tRNA genes showed the typical cloverleaf secondary structure, except for trnSer, which did not have the dihydrouridine arm. Furthermore, the secondary structures of rRNAs (rrnL and rrnS) in O. ritcheriina were predicted. Overall, five domains and 42 helices were predicted for rrnL (domain III is absent in arthropods), and three structural domains and 27 helices were predicted for rrnS. Maximum likelihood and Bayesian analyses indicated that O. ritcheriina and other Coelidiinae members were clustered into a clade, indicating the relationships among their subfamilies; the main topology was as follows: (Deltocephalinae + ((Coelidiinae + Iassinae) + ((Typhlocybinae + Cicadellinae) + (Idiocerinae + (Treehopper + Megophthalminae))))). The phylogenetic relationships indicated that the molecular taxonomy of O. ritcheriina is consistent with the current morphological classification.

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The mitogenome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae).

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